Determination of dissolved deoxyribonucleic acid concentration in lake water
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چکیده
In this paper we d~scuss the sultablllty of the cetyltrimethylammonium bromlde (CTAB) precipitation method for the determination of dissolved DNA (dDNA) In lake water. Factors affecting dDNA recovery from the precipitate and further DAPI fluorometr~c assay were also examined. The proposed method was particularly suitable for routine determination of dDNA concentration in low and moderately eutrophicated freshivater environments The detection 11m1t of the method vaned from 1.5 pg dDNA 1.' for mesotrophlc waters to 2 5 pg dDNA I' for eu t roph~c lakes. Preliminary observatlons on the concentration of dDNA in the surface water of 8 southern German lakes showed that dDNA concentrations were generally related to their trophic status and varied between 2.5 and 46.0 pg I' in mesotrophlc and from 11.5 to 72 pg I-' in eutrophlc waters K E Y WORDS. Dissolved D N A . Lake wa te r . CTAB . DAPI
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